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Amazon Drive Cloud storage from Amazon. Shopbop Designer Fashion Brands. Warehouse Deals Open-Box Discounts. There are three related Hoechst stains: Hoechst , Hoechst , and Hoechst The dyes Hoechst and Hoechst are the ones most commonly used and they have similar excitation — emission spectra.

Hoechst stains can be excited with a xenon- or mercury-arc lamp or with an ultraviolet laser. There is a considerable Stokes shift between the excitation and emission spectra that makes Hoechst dyes useful in experiments in which multiple fluorophores are used.

The fluorescence intensity of Hoechst dyes also increases with the pH of the solvent. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide.

The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine and thymine.

Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Thus, these stains are often called supravital , meaning that live cells survive a treatment with these compounds.

Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.

A concentration of 0. A green fluorescence of unbound Hoechst dye may be observed on samples which are stained with too much dye or which are washed partially.

Hoechst and are quenched by bromodeoxyuridine BrdU , which is commonly used to detect dividing cells. Hoechst exhibits a 10 fold greater cell-permeability than H Have a question?

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